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1 | 1 | # Finding, Optimizing, and Reporting Cas Targets (FORCAST): a fully integrated and open source pipeline to design CRISPR mutagenesis experiments
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2 | 2 |
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3 | 3 | ## Contents
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4 |
| -[Citation](#citation]<br> |
5 | 4 | [Hardware Requirements](#hardware-requirements)<br>
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6 | 5 | [Installing With Docker *\*(RECOMMENDED)\**](#installing-with-docker)<br>
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7 | 6 | [Installing Natively](#installing-natively)<br>
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8 | 7 | [Customization](#customization)<br>
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9 | 8 | [Migration](#migration)<br>
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| 9 | +[Citation](#citation)<br> |
10 | 10 | [Troubleshooting](#troubleshooting)<br>
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11 | 11 |
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12 |
| -## Citation |
13 |
| - |
14 |
| -<a href="https://doi.org/10.1101/2020.04.21.053090"> |
15 |
| - |
16 |
| -``` |
17 |
| -FORCAST: a fully integrated and open source pipeline to design Cas-mediated mutagenesis experiments |
18 |
| -Hillary Elrick, Viswateja Nelakuditi, Greg Clark, Michael Brudno, Arun K. Ramani, Lauryl M.J. Nutter |
19 |
| -bioRxiv 2020.04.21.053090; doi: https://doi.org/10.1101/2020.04.21.053090 |
20 |
| -``` |
21 |
| -</a> |
22 |
| - |
23 | 12 | ## Hardware Requirements
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24 | 13 | FORCAST can be installed on any machine running a [docker-compatible operating system](https://docs.docker.com/install/). Alternately, to run FORCAST natively, Ubuntu 16.04 is required.
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25 | 14 |
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@@ -189,6 +178,17 @@ sudo python customPython/MongoHandler.py mm10
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189 | 178 |
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190 | 179 | Your existing Primer and Guide designs should now be accessible in the new FORCAST installation!
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191 | 180 |
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| 181 | +## Citation |
| 182 | + |
| 183 | +<a href="https://doi.org/10.1101/2020.04.21.053090"> |
| 184 | + |
| 185 | +``` |
| 186 | +FORCAST: a fully integrated and open source pipeline to design Cas-mediated mutagenesis experiments |
| 187 | +Hillary Elrick, Viswateja Nelakuditi, Greg Clark, Michael Brudno, Arun K. Ramani, Lauryl M.J. Nutter |
| 188 | +bioRxiv 2020.04.21.053090; doi: https://doi.org/10.1101/2020.04.21.053090 |
| 189 | +``` |
| 190 | +</a> |
| 191 | + |
192 | 192 | ## Troubleshooting
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193 | 193 |
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194 | 194 | If you encounter issues installing or using FORCAST, raise a GitHub issue or start a GitHub discussion.
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