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README.md

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# Finding, Optimizing, and Reporting Cas Targets (FORCAST): a fully integrated and open source pipeline to design CRISPR mutagenesis experiments
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## Contents
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[Citation](#citation]<br>
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[Hardware Requirements](#hardware-requirements)<br>
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[Installing With Docker *\*(RECOMMENDED)\**](#installing-with-docker)<br>
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[Installing Natively](#installing-natively)<br>
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[Customization](#customization)<br>
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[Migration](#migration)<br>
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[Citation](#citation)<br>
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[Troubleshooting](#troubleshooting)<br>
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## Citation
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<a href="https://doi.org/10.1101/2020.04.21.053090">
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```
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FORCAST: a fully integrated and open source pipeline to design Cas-mediated mutagenesis experiments
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Hillary Elrick, Viswateja Nelakuditi, Greg Clark, Michael Brudno, Arun K. Ramani, Lauryl M.J. Nutter
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bioRxiv 2020.04.21.053090; doi: https://doi.org/10.1101/2020.04.21.053090
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```
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</a>
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## Hardware Requirements
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FORCAST can be installed on any machine running a [docker-compatible operating system](https://docs.docker.com/install/). Alternately, to run FORCAST natively, Ubuntu 16.04 is required.
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Your existing Primer and Guide designs should now be accessible in the new FORCAST installation!
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## Citation
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<a href="https://doi.org/10.1101/2020.04.21.053090">
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```
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FORCAST: a fully integrated and open source pipeline to design Cas-mediated mutagenesis experiments
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Hillary Elrick, Viswateja Nelakuditi, Greg Clark, Michael Brudno, Arun K. Ramani, Lauryl M.J. Nutter
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bioRxiv 2020.04.21.053090; doi: https://doi.org/10.1101/2020.04.21.053090
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```
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</a>
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## Troubleshooting
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If you encounter issues installing or using FORCAST, raise a GitHub issue or start a GitHub discussion.

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